Wuhan Healthy Baby Cohort Study is a population-based prospective cohort study from fetal life until adulthood. The study is designed to identify early environmental and genetic causes and causal pathways leading to normal and abnormal growth, development and health from fetal life, childhood and young adulthood. This multidisciplinary study focuses on several health outcomes including pregnant complications, adverse neonatal birth outcomes, childbehavior and neuro-cognition, body composition, growth, hearing, heart and vascular development, infectious disease and immunity, respiratory health, allergy and skin disorders of children and their parents. Main exposures of interest include environmental, endocrine, genomic (genetic, epigenetic, microbiome), lifestyle related, nutritional and socio-demographic determinants. In total, 11311 mothers with a delivery date from April 2012 until September 2014, and 10160 mothers from early pregnancy of January2014until October 2019 were enrolled in the study. Response at baseline was 95%, and general follow-up rates among children until the age of 3 years were around 80%. Data collection in children and their parents includes questionnaires, interviews, detailed physical and ultrasound examinations, behavioral observations, neurodevelopment evaluation and biological sampling. Genome and epigenome wide association screens are available. By the end of 2021, More than 160 original research papers have been published, most of them are published in high impact journals, including Lancet Planet Health，Circ Res，Environ Health Prospect，Environ Int0，BMC Med etc.furthermore, over 15 research grants were funded by NSF of China with a total amount of funding over 3 million USD.Eventually, results from the Wuhan Healthy Baby Cohort Study contribute to the development of strategies for optimizing health and healthcare for pregnant women and children.